What is Structured Illumination Microscopy? Structured Illumination Microscopy (SIM) is a super-resolution fluorescence microscopy technique that allows for imaging beyond the diffraction limit of ...

Scientists at EPFL have published a guide to building an add-on that turns a standard optical microscope into an instrument capable of producing super resolution, 3D images of cells, organoids, and ...

Structured illumination microscopy (SIM) is the most preferable system for live-cell super-resolution imaging. It enables the observation of intricate subcellular dynamics. However, conventional SIM ...

SIMIP enables high-resolution images rich in both chemical and spatial information. A quantum cascade laser (QCL) excites molecular vibrations while a spatial light modulator (SLM) generates striped ...

Top: Random Illumination Microscopy (RIM) utilizes random speckle patterns to achieve super-resolution imaging of biological tissues, improving resolution by a factor of two compared to the optical ...

Researchers used structured light and switchable fluorescent molecules to reduce the background light from the out-of-plane regions of microscope samples. This method allowed for the acquisition of ...

Even those who maintain that super-resolution microscopy is a powerful tool of biological discovery have admitted that it may have a bit of an image problem. For example, in a recent review, several ...

A project at Swiss research center EPFL has designed an add-on unit intended to bring structured light capability to a standard optical microscope. Described in Nature Communications, the design ...

More information: Pengcheng Fu et al, Breaking the diffraction limit in molecular imaging by structured illumination mid-infrared photothermal microscopy, Advanced Photonics (2025). DOI: ...

This is especially true when studying intricate details beyond the optical resolution limit using structured illumination microscopy (SIM). SIM uses multiple images acquired under structured ...